Fast Ligation-Mediated PCR, a Fast and Reliable Method for IS 6110 -Based Typing of Mycobacterium tuberculosis Complex
نویسندگان
چکیده
منابع مشابه
Fast ligation-mediated PCR, a fast and reliable method for IS6110-based typing of Mycobacterium tuberculosis complex.
IS6110 restriction fragment length polymorphism (RFLP) analysis is the most widely applied method for strain differentiation of Mycobacterium tuberculosis complex. We have previously described mixed-linker PCR, an IS6110-based PCR method that favorably compared with other typing methods for M. tuberculosis complex according to reproducibility and ability to differentiate between strains. Here w...
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Restriction fragment length polymorphism (RFLP) based on the insertion sequence IS6110 is used to investigate episodes of suspected transmission of infection of tuberculosis but usually takes a number of weeks from receipt of request to obtain a result. Often investigations would benefit from a more rapid method, possibly one containing an amplification step. The method employed uses a simple D...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2005
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.43.11.5622-5627.2005